The nail plate constitutes a formidable barrier to topical therapy, often leading to suboptimal treatment outcomes in onychomycosis. This thesis explores the combined use of fractional CO₂ laser ablation and extended-release formulations to enhance ungual drug delivery. Terbinafine and amphotericin B were selected as model antifungals to address dermatophyte and non-dermatophyte infections, respectively. Nanocrystal-based and liposomal formulations embedded in thermosensitive gels were developed, characterised, and evaluated ex vivo using porcine hooves as nail models. Fractional laser ablation generated reproducible micropores that improved drug deposition and enabled sustained release from the formulations. Terbinafine nanocrystals achieved a 22-fold increase in deposition compared to commercial cream, while amphotericin B liposomes demonstrated enhanced nail penetration despite limited antifungal efficacy. The findings support the potential of integrating controlled-release strategies with laser-assisted drug delivery to overcome nail permeability barriers, improve patient compliance, and provide more effective topical treatments for onychomycosis.