The carbodiimide (EDC)-induced catalytic conversion of linear triphosphate to cyclic trimetaphosphate was spectrally signalized by ³¹P NMR at -21.5 ppm. Further enhancement of this peak in the additional presence of either imidazole or adenine (also other nucleobases) was interpreted as resulting of the opening of the trimetaphosphate ring by their phosphoramidate bonding followed by polymerization to linear polybasephosphates also signalized at -21.5 ppm.