Simple kinetic determination of trace amounts of ascorbic acid in plant extracts

Celardin, Fredi; Castillo, Federico; Greppin, Hubert

doi:10.1016/0165-022X(82)90054-9

Scientific article

English

Simple kinetic determination of trace amounts of ascorbic acid in plant extracts

ContributorsCelardin, Fredi; Castillo, Federico; Greppin, Hubert

Published inJournal of biochemical and biophysical methods, vol. 6, no. 2, p. 89-93

Publication date1982

Abstract

A kinetic method of ascorbic acid assay based on the lag produced during the evolution of horse radish peroxidase-catalyzed oxidation of guaiacol or of homovanillic acid by hydrogen peroxide, is described and applied to ascorbic acid determination in Sedum album extracts.

Keywords

Ascorbic acid
Peroxidase
Kinetic analysis

Affiliation entities

Faculté des sciences / Section de biologie / Département des sciences végétales

Citation (ISO format)

CELARDIN, Fredi, CASTILLO, Federico, GREPPIN, Hubert. Simple kinetic determination of trace amounts of ascorbic acid in plant extracts. In: Journal of biochemical and biophysical methods, 1982, vol. 6, n° 2, p. 89–93. doi: 10.1016/0165-022X(82)90054-9