Scientific article
Open access

Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas

Published inPlant biotechnology journal, vol. 9, no. 5, p. 565-574
Publication date2011

Chloroplast transformation in microalgae offers great promise for the production of proteins of pharmaceutical interest or for the development of novel biofuels. For many applications, high level expression of transgenes is desirable. We have transformed the chloroplast of Chlamydomonas reinhardtii with two genes, acrV and vapA, which encode antigens from the fish pathogen Aeromonas salmonicida. The promoters and 5' untranslated regions of four chloroplast genes were compared for their ability to drive expression of the bacterial genes. The highest levels of expression were obtained when they were placed under the control of the cis-acting elements from the psaA-exon1 gene. The expression of these chimeric genes was further increased when a nuclear mutation that affects a factor involved in psaA splicing was introduced in the genetic background of the chloroplast transformants. Accumulation of both the chimeric mRNAs and the recombinant proteins was dramatically increased, indicating that negative feedback loops limit the expression of chloroplast transgenes. Our results demonstrate the potential of manipulating anterograde signalling to alter negative regulatory feedback loops in the chloroplast and improve transgene expression.

  • Aeromonas salmonicida/genetics/immunology
  • Antigens, Bacterial/genetics
  • Cell Nucleus/genetics
  • Chlamydomonas reinhardtii/genetics/metabolism
  • Chloroplasts/genetics/metabolism
  • Gene Expression Regulation, Bacterial
  • Gene Expression Regulation, Plant
  • Genetic Engineering/methods
  • Mutation
  • Plants, Genetically Modified/genetics/metabolism
  • Recombinant Proteins/biosynthesis/genetics
  • Transformation, Genetic
  • Transgenes
Citation (ISO format)
MICHELET, Laure et al. Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas. In: Plant biotechnology journal, 2011, vol. 9, n° 5, p. 565–574. doi: 10.1111/j.1467-7652.2010.00564.x
Main files (1)
Article (Accepted version)
ISSN of the journal1467-7644

Technical informations

Creation02/10/2012 3:11:00 PM
First validation02/10/2012 3:11:00 PM
Update time03/14/2023 5:08:15 PM
Status update03/14/2023 5:08:15 PM
Last indexation10/18/2023 1:21:20 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack