• Home
  1. Home
  2. HaCaT cells as a model system to study primary cilia in keratinocytes
Scientific article
OA Policy
English

HaCaT cells as a model system to study primary cilia in keratinocytes

Contributors

Blanchard, Gabriela; Pich, Christine; Hohl, Daniel

Published in

Experimental dermatology, vol. 31, no. 8, p. 1276-1280

Publication date

2022-08

First online date

2022-06-23

Abstract

Primary cilium (PC) is a microtubule-based organelle found on the apical surface of most mammalian cell types, playing a role in development and tissue homeostasis. Ciliopathies are a rapidly growing group of human diseases characterized by disordered cilium. PC plays an important role in pathogenesis of basal cell cancer, the most common human malignancy. A significant increase in ciliation has been observed in the epidermis of atopic dermatitis and psoriasis patients. Spontaneously immortalized human keratinocytes, HaCaT are a model to study the epidermal homeostasis and pathophysiology. In contrast to what has been previously described, here, we show that HaCaT can be efficiently ciliated. In HaCaT cells, differentiation significantly increased the number of ciliated cells and we were able to analyse in detail the ciliary length progression with duration of differentiation. As the number of recognized ciliopathies continues to increase, the importance of ciliary models also rises. Even though keratinocytes do not become as highly and rapidly ciliated as cell lines frequently used in ciliary studies, they are a better model for the study of skin ciliopathies. Detailed progression of ciliation in HaCaT could serve as the basis for ciliary studies in this cell line.

Keywords

  • Cancer
  • Differentiation
  • Keratinocyte biology
  • Keratinocytes
  • Primary cilium
  • Animals
  • Cilia / metabolism
  • Ciliopathies / metabolism
  • Epidermis
  • HaCaT Cells
  • Humans
  • Keratinocytes / metabolism
  • Mammals

UNIGE affiliation entities

Not a UNIGE publication

UNIGE research groups

Funding

Citation (ISO format)

BLANCHARD, Gabriela, PICH, Christine, HOHL, Daniel. HaCaT cells as a model system to study primary cilia in keratinocytes. In: Experimental dermatology, 2022, vol. 31, n° 8, p. 1276–1280. doi: 10.1111/exd.14626

Main files (1)

Article (Published version)
CC BY-NC-4.0

Identifiers

Additional URL for this publication

https://onlinelibrary.wiley.com/doi/10.1111/exd.14626

Journal ISSN

0906-6705
46views
113downloads

Technical informations

Creation

22/01/2025 09:28:06

First validation

25/02/2025 10:37:10

Update

16/06/2026 12:28:18

Status update

16/06/2026 12:28:18

Last indexation

16/06/2026 12:28:19
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated