Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Guerrero-Ferreira, Ricardo C; Viollier, Patrick; Ely, Bert; Poindexter, Jeanne S; Georgieva, Maria; Jensen, Grant J; Wright, Elizabeth R

doi:10.1073/pnas.1012388108

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Title		Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus
Authors		Guerrero-Ferreira, Ricardo C Viollier, Patrick Ely, Bert Poindexter, Jeanne S Georgieva, Maria Jensen, Grant J Wright, Elizabeth R
Published in		Proceedings of the National Academy of Sciences. 2011, vol. 108, no. 24, p. 9963-8
Abstract		2D and 3D cryo-electron microscopy, together with adsorption kinetics assays of Cb13 and CbK phage-infected Caulobacter crescentus, provides insight into the mechanisms of infection. Cb13 and CbK actively interact with the flagellum and subsequently attach to receptors on the cell pole. We present evidence that the first interaction of the phage with the bacterial flagellum takes place through a filament on the phage head. This contact with the flagellum facilitates concentration of phage particles around the receptor (i.e., the pilus portals) on the bacterial cell surface, thereby increasing the likelihood of infection. Phage head filaments have not been well characterized and their function is described here. Phage head filaments may systematically underlie the initial interactions of phages with their hosts in other systems and possibly represent a widespread mechanism of efficient phage propagation.
Keywords		Bacterial Adhesion — Bacteriophages/physiology/ultrastructure — Caulobacter crescentus/genetics/ultrastructure/virology — Cryoelectron Microscopy/methods — Fimbriae, Bacterial/genetics/ultrastructure — Flagella/genetics/ultrastructure/virology — Host-Pathogen Interactions — Imaging, Three-Dimensional/methods — Models, Biological — Mutation
Identifiers		DOI: 10.1073/pnas.1012388108 PMID: 21613567
Full text		Article (Accepted version) (945 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Citation (ISO format)		GUERRERO-FERREIRA, Ricardo C et al. Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus. In: Proceedings of the National Academy of Sciences, 2011, vol. 108, n° 24, p. 9963-8. doi: 10.1073/pnas.1012388108 https://archive-ouverte.unige.ch/unige:18256