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Scientific article
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English

In vitro engineering of bone using a rotational oxygen-permeable bioreactor system

Contributors

Terai, Hidetomi; Hannouche, Didier; Ochoa, Erin; Yamano, Yoshiki; Vacanti, Joseph P.

Published in

Materials science & engineering. C, Biomimetic materials, sensors and systems, vol. 20, no. 1-2, p. 3-8

Publication date

2002-05

Abstract

Tissue engineering of bone may supersede the need in the future of autograft procedures to treat bone defects resulting from trauma or developmental diseases. A Rotational Oxygen-Permeable Bioreactor System (ROBS) has recently been developed in our laboratory to reproduce dynamic and gas-permeable culture conditions that would supply optimal oxygen and continuous loading to cell/polymer constructs in culture. The cell culture media in ROBS were examined at 1, 24 and 48 h to evaluate the kinetics of pO2, pCO2 and pH without culturing cells. The results were compared to the kinetics in 100 mm diameter cell culture dishes (Control I: static, gas permeable) and 50 ml centrifuge tubes (Control II: dynamic, non-gas permeable). The results showed the same kinetics in ROBS and Control I, whereas Control II failed to maintain the gas conditions of the media. Next, osteoblasts derived from mesenchymal stromal cells (MSCs) of neonatal rats were cultured in three-dimensional poly(dl-lactide-co-glycolide) (PLGA) foams using ROBS to study the effectiveness of this bioreactor system to support cell growth and differentiation. Mineralization was observed within 2 weeks of culture and was shown throughout the polymer at 7 weeks with embedded osteocytic cells. This study demonstrates the usefulness of ROBS for in vitro bone tissue engineering.

Keywords

  • Bioreactor
  • Tissue engineering
  • Bone
  • In vitro
  • Partial gas pressure

UNIGE affiliation entities

Not a UNIGE publication

UNIGE research groups

Citation (ISO format)

TERAI, Hidetomi et al. In vitro engineering of bone using a rotational oxygen-permeable bioreactor system. In: Materials science & engineering. C, Biomimetic materials, sensors and systems, 2002, vol. 20, n° 1-2, p. 3–8. doi: 10.1016/S0928-4931(02)00006-1

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Article (Published version)

Identifiers

Additional URL for this publication

https://linkinghub.elsevier.com/retrieve/pii/S0928493102000061

Journal ISSN

0928-4931
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100downloads

Technical informations

Creation

17/11/2024 19:38:32

First validation

19/11/2024 13:53:08

Update

30/04/2026 10:44:32

Status update

30/04/2026 10:44:32

Last indexation

30/04/2026 10:46:46
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