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Interleukin (IL)-33 induces the release of pro-inflammatory mediators by mast cells

Moulin, David
Published in Cytokine. 2007, vol. 40, no. 3, p. 216-25
Abstract IL-33 (or IL-1F11) was recently identified as a ligand for the previously orphaned IL-1 family receptor T1/ST2. Previous studies have established that IL-33 and T1/ST2 exert key functions in Th2 responses. In this study, we demonstrate that IL-33 induces the production of pro-inflammatory mediators in mast cells. IL-33 dose and time-dependently stimulated IL-6 secretion by P815 mastocytoma cells and primary mouse bone marrow-derived mast cells (BMMC). This effect was dependent on T1/ST2 binding. In addition, IL-33 also induced IL-1beta, TNF-alpha, MCP-1, and PGD2 production in BMMC. By RNase protection assay, we demonstrated that IL-33 increased IL-6 and IL-1beta mRNA expression. These effects of IL-33 appeared to occur independently of mast cell degranulation, The results of this study show for the first time that IL-33, a novel member of the IL-1 family of cytokines, stimulates the production of pro-inflammatory mediators by mast cells in addition to its effect on T helper 2 responses. These findings open new perspectives for the treatment of inflammatory diseases by targeting IL-33.
Keywords AnimalsBone Marrow Cells/cytology/immunology/metabolismCell LineChemokine CCL2/biosynthesis/immunologyDose-Response Relationship, DrugInflammation/drug therapy/immunology/metabolismInflammation Mediators/immunology/metabolismInterleukin-1beta/biosynthesis/immunologyInterleukins/immunology/pharmacologyMast Cells/cytology/immunology/metabolismMembrane Proteins/immunology/metabolismMiceProstaglandin D2/biosynthesis/immunologyProtein Binding/immunologyTh2 Cells/cytology/immunologyTime FactorsTumor Necrosis Factor-alpha/biosynthesis/immunology
PMID: 18023358
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Research group Mécanisme de l'inflammation articulaire (44)
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MOULIN, David et al. Interleukin (IL)-33 induces the release of pro-inflammatory mediators by mast cells. In: Cytokine, 2007, vol. 40, n° 3, p. 216-25. https://archive-ouverte.unige.ch/unige:1814

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Deposited on : 2009-05-28

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