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Cytotoxicity of endodontic materials over 6-weeks ex vivo

Brackett, Martha Goël
Marshall, A.
Lockwood, Petra E.
Lewis, Jill B.
Messer, Regina L W.
Wataha, John C.
Published in International endodontic journal. 2008, vol. 41, no. 12, p. 1072-8
Abstract AIM: To test the hypothesis that extending the time of a traditional ex vivo cytotoxicity test helps to identify trends in the behaviour of root core materials and sealers, which could ultimately aid in predicting their clinical safety and performance. METHODOLOGY: Endodontic sealers and core specimens were initially tested in direct contact with L929 fibroblasts for 72 h. Cell response was estimated by measuring cellular succinate dehydrogenase activity relative to Teflon controls. Cytotoxicity (% of more active cells) was reassessed after 1, 3, 4 and 6 weeks, with the specimens stored in a physiologically balanced salt-solution between tests. RESULTS: Distinct trends in cytotoxicity among both core materials and sealers were observed over the 6-week test. Four of the six sealers and two of the three core materials showed cell viabilities of <30% of Teflon after 6 weeks (>70% cytotoxicity). CONCLUSIONS: The current results suggest that some endodontic materials have an elevated biological risk for extended intervals.
Keywords AnimalsCell LineCell Survival/drug effectsColoring Agents/diagnostic useComposite Resins/toxicityDimethylpolysiloxanes/toxicityDrug CombinationsEpoxy Resins/toxicityFibroblasts/drug effectsForecastingGutta-Percha/toxicityMaterials TestingMiceMitochondria/enzymologyPolyesters/toxicityResin Cements/toxicityRoot Canal Filling Materials/toxicitySafetySuccinate Dehydrogenase/drug effectsTetrazolium Salts/diagnostic useThiazoles/diagnostic useTime FactorsZinc Oxide-Eugenol Cement/toxicity
PMID: 19133096
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Research group Groupe Bouillaguet Serge (medecine dentaire) (846)
(ISO format)
BRACKETT, Martha Goël et al. Cytotoxicity of endodontic materials over 6-weeks ex vivo. In: International endodontic journal, 2008, vol. 41, n° 12, p. 1072-8. doi: 10.1111/j.1365-2591.2008.01471.x https://archive-ouverte.unige.ch/unige:1813

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Deposited on : 2009-05-28

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