en
Scientific article
English

Rapid identification of acetic acid bacteria using MALDI-TOF mass spectrometry fingerprinting

Published inSystematic and applied microbiology, vol. 36, no. 2, p. 75-81
Publication date2013-03
Abstract

Acetic acid bacteria (AAB) are widespread microorganisms characterized by their ability to transform alcohols and sugar-alcohols into their corresponding organic acids. The suitability of matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF MS) for the identification of cultured AAB involved in the industrial production of vinegar was evaluated on 64 reference strains from the genera Acetobacter, Gluconacetobacter and Gluconobacter. Analysis of MS spectra obtained from single colonies of these strains confirmed their basic classification based on comparative 16S rRNA gene sequence analysis. MALDI-TOF analyses of isolates from vinegar cross-checked by comparative sequence analysis of 16S rRNA gene fragments allowed AAB to be identified, and it was possible to differentiate them from mixed cultures and non-AAB. The results showed that MALDI-TOF MS analysis was a rapid and reliable method for the clustering and identification of AAB species.

eng
Keywords
  • MALDI-TOF MS
  • Whole-cell identification
  • Acetic acid bacteria
  • Vinegar
Citation (ISO format)
ANDRES BARRAO, Cristina et al. Rapid identification of acetic acid bacteria using MALDI-TOF mass spectrometry fingerprinting. In: Systematic and applied microbiology, 2013, vol. 36, n° 2, p. 75–81. doi: 10.1016/j.syapm.2012.09.002
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0723-2020
10views
0downloads

Technical informations

Creation06/09/2024 3:40:02 PM
First validation06/14/2024 10:25:47 AM
Update time06/14/2024 10:25:47 AM
Status update06/14/2024 10:25:47 AM
Last indexation06/14/2024 10:26:08 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack