Scientific article

Rapid identification of acetic acid bacteria using MALDI-TOF mass spectrometry fingerprinting

Published inSystematic and applied microbiology, vol. 36, no. 2, p. 75-81
Publication date2013-03

Acetic acid bacteria (AAB) are widespread microorganisms characterized by their ability to transform alcohols and sugar-alcohols into their corresponding organic acids. The suitability of matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF MS) for the identification of cultured AAB involved in the industrial production of vinegar was evaluated on 64 reference strains from the genera Acetobacter, Gluconacetobacter and Gluconobacter. Analysis of MS spectra obtained from single colonies of these strains confirmed their basic classification based on comparative 16S rRNA gene sequence analysis. MALDI-TOF analyses of isolates from vinegar cross-checked by comparative sequence analysis of 16S rRNA gene fragments allowed AAB to be identified, and it was possible to differentiate them from mixed cultures and non-AAB. The results showed that MALDI-TOF MS analysis was a rapid and reliable method for the clustering and identification of AAB species.

  • Whole-cell identification
  • Acetic acid bacteria
  • Vinegar
Citation (ISO format)
ANDRES BARRAO, Cristina et al. Rapid identification of acetic acid bacteria using MALDI-TOF mass spectrometry fingerprinting. In: Systematic and applied microbiology, 2013, vol. 36, n° 2, p. 75–81. doi: 10.1016/j.syapm.2012.09.002
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Article (Published version)
ISSN of the journal0723-2020

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