Scientific article
Open access

Determination of the pH Gradient Across the Stratum Corneum

Publication date1998-08

The objective of this work was to determine the pH gradient profile across hairless mouse stratum corneum (SC) using ratiometric laser scanning confocal microscopy imaging and direct pH measurement with a flat surface electrode. Dual-emission ratiometric imaging used the fluorophore, carboxy seminaphthorhodafluor-1, which displays a pH-dependent shift in its emission spectrum. The assay developed was unsuccessful, however, because (i) the pKa of seminaphthorhodafluor (approximately 7.5) makes it insufficiently sensitive to the normal pH gradient (4-7.4) across mammalian SC, and (ii) the unexpectedly high buffering capacity of the skin precluded a meaningful calibration of the system. In the second method, pH measurements with a flat surface electrode were recorded as the hairless mouse SC was progressively tape stripped. In the superficial SC, the pH remained relatively constant (approximately 5.9); further removal of the barrier resulted in a steady increase in pH to approximately 7. In conclusion, improved methodology is clearly required to characterize precisely the pH profile across the SC.

  • Skin pH
  • Confocal microscopy
  • Stratum corneum
  • Barrier function
Affiliation Not a UNIGE publication
Citation (ISO format)
TURNER, Norris G., CULLANDER, Christopher, GUY, Richard H. Determination of the pH Gradient Across the Stratum Corneum. In: The Journal of investigative dermatology symposium proceedings, 1998, vol. 3, n° 2, p. 110–113. doi: 10.1038/jidsymp.1998.23
Main files (1)
Article (Published version)
ISSN of the journal1087-0024

Technical informations

Creation06/04/2024 8:37:06 AM
First validation06/10/2024 7:54:22 AM
Update time06/10/2024 7:54:22 AM
Status update06/10/2024 7:54:22 AM
Last indexation06/10/2024 7:54:43 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack