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Scientific article
English

Endothelial Dysfunction in Murine Model of Systemic Sclerosis: Tight-skin Mice 1

Published inJournal of investigative dermatology, vol. 119, no. 6, p. 1379-1387
Publication date2002-12
Abstract

We conducted this study to analyze endothelial cell function within intact thoracic aorta of the systemic sclerosis murine model, the heterozygous tight-skin mice 1: (i) assessing the distribution and activation intensity of endothelial cells, responsive to endothelium-dependent vasodilators (acetylcholine, adenosine triphosphate, bradykinin, and substance P) and Iloprost, using laser line confocal microscopy in combination with two Ca2+ fluorescent dyes; (ii) evaluating en-dothelium-dependent vasodilator- and Iloprostinduced relaxation, using isometric tension measurement; and (iii) investigating the role of nitric oxide in mediating relaxation to acetylcholine and adenosine triphosphate. The number of activated endothelial cells was significantly lower in heterozygous tight-skin mice 1, compared with controls, for adenosine triphosphate and Iloprost. Maximal increase of Ca2+ fluorescence intensity ratio in activated endothelial cells was decreased for adenosine triphosphate, bradykinin, and Iloprost, in heterozygous tight-skin mice 1. Adenosine triphosphate- and Iloprost-mediated aortic relaxation was further impaired in heterozygous tight-skin mice 1. Finally, aortic relaxation to acetylcholine and adenosine triphosphate was markedly decreased by nitric oxide synthase inhibitor in heterozygous tight-skin mice 1. This study suggests that endothelial cell receptors for endothelium-dependent vasodilators and Iloprost may not be homogeneously distributed or continuously expressed in thoracic aorta of heterozygous tight-skin mice 1, resulting in endothelium-dependent vasodilatation dysfunction

eng
Keywords
  • Endothelium-dependent relaxation
  • Nitric oxide
  • Prostacyclin analog
  • Systemic sclerosis
  • Tight-skin mice
Citation (ISO format)
MARIE, Isabelle, BENY, Jean-Louis. Endothelial Dysfunction in Murine Model of Systemic Sclerosis: Tight-skin Mice 1. In: Journal of investigative dermatology, 2002, vol. 119, n° 6, p. 1379–1387. doi: 10.1046/j.1523-1747.2002.19614.x
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ISSN of the journal0022-202X
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