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Scientific article
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English

Understanding protein-nanoparticle interactions leading to protein corona formation: In vitro - in vivo correlation study

Published inInternational journal of biological macromolecules, vol. 256, no. P1, 128339
Publication date2024-01
Abstract

Nanoparticles (NPs) in contact with biological fluids form a biomolecular corona through interactions with proteins, lipids, and sugars, acquiring new physicochemical properties. This work explores the interaction between selected proteins (hemoglobin and fetuin-A) that may alter NP circulation time and NPs of different surface charges (neutral, positive, and negative). The interaction with key proteins albumin and transferrin, the two of the most abundant proteins in plasma was also studied. Binding affinity was investigated using quartz crystal microbalance and fluorescence quenching, while circular dichroism assessed potential conformational changes. The data obtained from in vitro experiments were compared to in vivo protein corona data. The results indicate that electrostatic interactions primarily drive protein-NP interactions, and higher binding affinity does not necessarily translate into more significant structural changes. In vitro and single protein-NP studies provide valuable insights that can be correlated with in vivo observations, opening exciting possibilities for future protein corona studies.

eng
Keywords
  • Binding affinity
  • Conformational changes
  • Nanoparticle
  • Protein corona
  • Protein structure
Research group
Funding
  • French National Research Agency (ANR) - Ingénierie et Innovation par les sciences physiques, les savoir-faire technologiques et l'interdisciplinarité [ANR-17-EURE-0002]
  • French National Research Agency (ANR) - Nanoparticles in blood: understanding and controlling protein corona for optimized nanomedicine [ANR-21-CE18-0015]
Citation (ISO format)
MARQUES, Cintia et al. Understanding protein-nanoparticle interactions leading to protein corona formation: <i>In vitro - in vivo</i> correlation study. In: International journal of biological macromolecules, 2024, vol. 256, p. 128339. doi: 10.1016/j.ijbiomac.2023.128339
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Article (Published version)
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Identifiers
ISSN of the journal0141-8130
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Creation02/20/2024 2:26:19 PM
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