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Scientific article
Open access
English

Use of the direct epifluorescent filter technique for the enumeration of viable and total acetic acid bacteria from vinegar fermentation

Published inJournal of fluorescence, vol. 13, no. 3, p. 261-265
Publication date2003
Abstract

A rapid epifluorescence staining method using the LIVE/DEAD® BacLight™ Bacterial Viability kit was applied to differentiate both viable and total counts of acetic acid bacteria in vinegar fermentation. The results obtained were compared with those from other measurement techniques: 4′,6-diamidine-2phenyl indole (DAPI) and 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and colony counts. BacLight total counts were comparable to DAPI (differing by <3.5%). BacLight viable counts were similar to CTC counts but considerable higher than colony-forming cells in plates.

eng
Keywords
  • Vinegar
  • Acetic acid bacteria
  • Acetators
  • BacLight
  • DAPI
  • CTC
  • Viability staining
Citation (ISO format)
FORERO MESA, Maria Del Mar et al. Use of the direct epifluorescent filter technique for the enumeration of viable and total acetic acid bacteria from vinegar fermentation. In: Journal of fluorescence, 2003, vol. 13, n° 3, p. 261–265. doi: 10.1023/A:1025094017265
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ISSN of the journal1053-0509
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