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Scientific article
Open access
English

Dual-view oblique plane microscopy (dOPM)

Published inBiomedical optics express, vol. 11, no. 12, p. 7204
Publication date2020-11-18
First online date2020-11-18
Abstract

We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.

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Affiliation Not a UNIGE publication
Funding
  • Wellcome Trust - Theoretical Physics of Biology Laboratory [FC001317]
  • Wellcome Trust - Adult Stem Cell Laboratory [FC001039]
  • UK Research and Innovation - Stochastic fluctuations during mammary development and breast cancer morphogenesis [EP/T003103/1]
  • Cancer Research UK - [C37275/A20146]
  • The Francis Crick Institute - [10317]
Citation (ISO format)
SPARKS, Hugh et al. Dual-view oblique plane microscopy (dOPM). In: Biomedical optics express, 2020, vol. 11, n° 12, p. 7204. doi: 10.1364/boe.409781
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ISSN of the journal2156-7085
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