en
Scientific article
Open access
English

The Drosophila ecdysone receptor promotes or suppresses proliferation according to ligand level

Published inDevelopmental cell, vol. 58, no. 20, p. 2128-2139.e4
Publication date2023-10
Abstract

The steroid hormone 20-hydroxy-ecdysone (20E) promotes proliferation in Drosophila wing precursors at low titer but triggers proliferation arrest at high doses. Remarkably, wing precursors proliferate normally in the complete absence of the 20E receptor, suggesting that low-level 20E promotes proliferation by overriding the default anti-proliferative activity of the receptor. By contrast, 20E needs its receptor to arrest proliferation. Dose-response RNA sequencing (RNA-seq) analysis of ex vivo cultured wing precursors identifies genes that are quantitatively activated by 20E across the physiological range, likely comprising positive modulators of proliferation and other genes that are only activated at high doses. We suggest that some of these "high-threshold" genes dominantly suppress the activity of the pro-proliferation genes. We then show mathematically and with synthetic reporters that combinations of basic regulatory elements can recapitulate the behavior of both types of target genes. Thus, a relatively simple genetic circuit can account for the bimodal activity of this hormone.

eng
Keywords
  • Drosophila
  • Ecdysone
  • Growth control
  • Hormone signaling
  • Model of transcriptional control
  • Nuclear hormone
  • Proliferation control
  • Wing imaginal discs
Funding
  • Wellcome Trust - Theoretical Physics of Biology Laboratory [FC001317]
  • Wellcome Trust - Epithelial Cell Interactions Laboratory [FC001204]
  • Cancer Research UK - [CC2072]
  • Medical Research Council -
  • European Molecular Biology Organization - [ALTF 238-2018]
  • The Francis Crick Institute - [CC2062]
Citation (ISO format)
PEREZ-MOCKUS, Gantas et al. The <i>Drosophila</i> ecdysone receptor promotes or suppresses proliferation according to ligand level. In: Developmental cell, 2023, vol. 58, n° 20, p. 2128–2139.e4. doi: 10.1016/j.devcel.2023.08.032
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal1534-5807
17views
3downloads

Technical informations

Creation05/04/2024 7:50:25 AM
First validation05/15/2024 12:22:22 PM
Update time05/15/2024 12:22:22 PM
Status update05/15/2024 12:22:22 PM
Last indexation05/15/2024 12:22:25 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack