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Book chapter
English

Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons

Published inCircadian clocks : methods and protocols, Editors Brown, Steven A., p. 207-219
PublisherNew York : Springer
Collection
  • Methods in molecular biology; 2130
Publication date2021-12-08
Abstract

Live imaging of the molecular clockwork within the circadian pacemaker neurons offers the unique possibility to study complex interactions between the molecular clock and neuronal communication within individual neurons and throughout the entire circadian circuitry. Here we describe how to establish brain explants and dissociated neuron culture from Drosophila larvae, guidelines for time-lapse fluorescence microscopy, and the method of image analysis. This approach enables the long-term monitoring of fluorescence signals of circadian reporters at single-cell resolution and can be also applicable to analyze real-time expression of other fluorescent probes in Drosophila neurons.

eng
Keywords
  • Drosophila dissociated neuronal culture
  • Drosophila whole brain explant culture
  • Time-lapse fluorescence microscopy
Citation (ISO format)
SABADO, Virginie, NAGOSHI, Emi. Fluorescence Live Imaging of <i>Drosophila</i> Circadian Pacemaker Neurons. In: Circadian clocks : methods and protocols. New York : Springer, 2021. p. 207–219. (Methods in molecular biology) doi: 10.1007/978-1-0716-0381-9_16
Main files (1)
Book chapter (Published version)
accessLevelRestricted
Identifiers
ISBN1071603817
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