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Book chapter
English

Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows

Published inZebrafish: methods and protocols, Editors Amatruda, James F. ; Houart, Corinne ; Kawakami, Koichi & Poss, Kenneth D., p. 185-204
PublisherNew York : Humana Press
EditionThird edition
Collection
  • Methods in molecular biology; 2707
Publication date2024-09-06
First online date2023-09-06
Abstract

In regeneration, a damaged body part grows back to its original form. Understanding the mechanisms and physical principles underlying this process has been limited by the difficulties of visualizing cell signals and behaviors in regeneration. Zebrafish scales are emerging as a model system to investigate morphogenesis during vertebrate regeneration using quantitative live imaging. Scales are millimeter-sized dermal bone disks forming a skeletal armor on the body of the fish. The scale bone is deposited by an adjacent monolayer of osteoblasts that, after scale loss, regenerates in about 2 weeks. This intriguing regenerative process is accessible to live confocal microscopy, quantifications, and mathematical modeling. Here, I describe methods to image scale regeneration live, tissue-wide and at sub-cellular resolution. Furthermore, I describe methods to process the resulting images and quantify cell, tissue, and signal dynamics.

eng
Keywords
  • Bone
  • Erk signaling
  • Morphogenesis
  • Physics of biology
  • Regeneration
  • Scale
  • Tissue growth
  • Tissue mechanics
  • Zebrafish
Citation (ISO format)
DE SIMONE, Alessandro. Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows. In: Zebrafish: methods and protocols. New York : Humana Press, 2024. p. 185–204. (Methods in molecular biology) doi: 10.1007/978-1-0716-3401-1_12
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Book chapter (Published version)
accessLevelRestricted
Identifiers
ISBN9781071634004
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Creation03/17/2024 10:03:14 AM
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