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High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples

Publié dansiScience, vol. 26, no. 4, p. 1-18; 106452
Date de publication2023-04
Résumé

Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the 'Fast Green' dye that provides unmatched visualization of collagen 3D network architecture, via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.

eng
Mots-clés
  • Cell biology
  • Microstructure
  • Molecular biology
Citation (format ISO)
TIMIN, Grigorii, MILINKOVITCH, Michel C. High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples. In: iScience, 2023, vol. 26, n° 4, p. 1–18. doi: 10.1016/j.isci.2023.106452
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Article (Published version)
Identifiants
ISSN du journal2589-0042
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Informations techniques

Création13/04/2024 07:54:49
Première validation29/04/2024 13:42:39
Heure de mise à jour29/04/2024 13:42:39
Changement de statut29/04/2024 13:42:39
Dernière indexation06/05/2024 18:28:55
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