Scientific article
Open access

Fluorescence circadian imaging reveals a pdf-dependent transcriptional regulation of the drosophila molecular clock

Published inScientific reports, vol. 7, no. 1, p. 1-13; 41560
Publication date2017-01-30
First online date2017-01-30

Circadian locomotor behaviour is controlled by a pacemaker circuit composed of clock-containing neurons. To interrogate the mechanistic relationship between the molecular clockwork and network communication critical to the operation of the Drosophila circadian pacemaker circuit, we established new fluorescent circadian reporters that permit single-cell recording of transcriptional and post-transcriptional rhythms in brain explants and cultured neurons. Live-imaging experiments combined with pharmacological and genetic manipulations demonstrate that the neuropeptide pigment-dispersing factor (PDF) amplifies the molecular rhythms via time-of-day- and activity-dependent upregulation of transcription from E-box-containing clock gene promoters within key pacemaker neurons. The effect of PDF on clock gene transcription and the known role of PDF in enhancing PER/TIM stability occur via independent pathways downstream of the PDF receptor, the former through a cAMP-independent mechanism and the latter through a cAMP-PKA dependent mechanism. These results confirm and extend the mechanistic understanding of the role of PDF in controlling the synchrony of the pacemaker neurons. More broadly, our results establish the utility of the new live-imaging tools for the study of molecular-neural interactions important for the operation of the circadian pacemaker circuit.

Citation (ISO format)
SABADO, Virginie et al. Fluorescence circadian imaging reveals a pdf-dependent transcriptional regulation of the <i>drosophila</i> molecular clock. In: Scientific reports, 2017, vol. 7, n° 1, p. 1–13. doi: 10.1038/srep41560
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Article (Published version)
ISSN of the journal2045-2322

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