Scientific article
Open access

Computer simulations reveal mechanisms that organize nuclear dynein forces to separate centrosomes

Published inMolecular biology of the cell, vol. 28, no. 23, p. 3165-3170
Publication date2017-11-07

Centrosome separation along the surface of the nucleus at the onset of mitosis is critical for bipolar spindle assembly. Dynein anchored on the nuclear envelope is known to be important for centrosome separation, but it is unclear how nuclear dynein forces are organized in an anisotropic manner to promote the movement of centrosomes away from each other. Here we use computational simulations of Caenorhabditis elegans embryos to address this fundamental question, testing three potential mechanisms by which nuclear dynein may act. First, our analysis shows that expansion of the nuclear volume per se does not generate nuclear dynein–driven separation forces. Second, we find that steric interactions between microtubules and centrosomes contribute to robust onset of nuclear dynein–mediated centrosome separation. Third, we find that the initial position of centrosomes, between the male pronucleus and cell cortex at the embryo posterior, is a key determinant in organizing microtubule aster asymmetry to power nuclear dynein–dependent separation. Overall our work reveals that accurate initial centrosome position, together with steric interactions, ensures proper anisotropic organization of nuclear dynein forces to separate centrosomes, thus ensuring robust bipolar spindle assembly.

Affiliation Not a UNIGE publication
Citation (ISO format)
DE SIMONE, Alessandro, GONCZY, Pierre. Computer simulations reveal mechanisms that organize nuclear dynein forces to separate centrosomes. In: Molecular biology of the cell, 2017, vol. 28, n° 23, p. 3165–3170. doi: 10.1091/mbc.E16-12-0823
Main files (1)
Article (Published version)
ISSN of the journal1059-1524

Technical informations

Creation03/22/2024 12:56:37 PM
First validation04/19/2024 1:29:20 PM
Update time04/19/2024 1:29:20 PM
Status update04/19/2024 1:29:20 PM
Last indexation05/06/2024 6:25:30 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack