en
Scientific article
English

Reconstruction and deconstruction of human somitogenesis in vitro

Published inNature, vol. 614, no. 7948, p. 500-508
Publication date2022-12-21
First online date2022-12-21
Abstract

The vertebrate body displays a segmental organization that is most conspicuous in the periodic organization of the vertebral column and peripheral nerves. This metameric organization is first implemented when somites, which contain the precursors of skeletal muscles and vertebrae, are rhythmically generated from the presomitic mesoderm. Somites then become subdivided into anterior and posterior compartments that are essential for vertebral formation and segmental patterning of the peripheral nervous system1-4. How this key somitic subdivision is established remains poorly understood. Here we introduce three-dimensional culture systems of human pluripotent stem cells called somitoids and segmentoids, which recapitulate the formation of somite-like structures with anteroposterior identity. We identify a key function of the segmentation clock in converting temporal rhythmicity into the spatial regularity of anterior and posterior somitic compartments. We show that an initial 'salt and pepper' expression of the segmentation gene MESP2 in the newly formed segment is transformed into compartments of anterior and posterior identity through an active cell-sorting mechanism. Our research demonstrates that the major patterning modules that are involved in somitogenesis, including the clock and wavefront, anteroposterior polarity patterning and somite epithelialization, can be dissociated and operate independently in our in vitro systems. Together, we define a framework for the symmetry-breaking process that initiates somite polarity patterning. Our work provides a platform for decoding general principles of somitogenesis and advancing knowledge of human development.

eng
Affiliation Not a UNIGE publication
Citation (ISO format)
MIAO, Yuchuan et al. Reconstruction and deconstruction of human somitogenesis in vitro. In: Nature, 2022, vol. 614, n° 7948, p. 500–508. doi: 10.1038/s41586-022-05655-4
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0028-0836
7views
0downloads

Technical informations

Creation03/17/2024 10:08:41 AM
First validation04/19/2024 12:26:46 PM
Update time04/19/2024 12:26:46 PM
Status update04/19/2024 12:26:46 PM
Last indexation05/06/2024 6:25:17 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack