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Scientific article
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English

Induction of kidney-related gene programs through co-option of SALL1 in mole ovotestes

Published inDevelopment, vol. 150, no. 17, dev201562
Publication date2023-09-01
First online date2023-09-01
Abstract

Changes in gene expression represent an important source of phenotypic innovation. Yet how such changes emerge and impact the evolution of traits remains elusive. Here, we explore the molecular mechanisms associated with the development of masculinizing ovotestes in female moles. By performing integrative analyses of epigenetic and transcriptional data in mole and mouse, we identified the co-option of SALL1 expression in mole ovotestes formation. Chromosome conformation capture analyses highlight a striking conservation of the 3D organization at the SALL1 locus, but an evolutionary divergence of enhancer activity. Interspecies reporter assays support the capability of mole-specific enhancers to activate transcription in urogenital tissues. Through overexpression experiments in transgenic mice, we further demonstrate the capability of SALL1 to induce kidney-related gene programs, which are a signature of mole ovotestes. Our results highlight the co-option of gene expression, through changes in enhancer activity, as a plausible mechanism for the evolution of traits.

eng
Keywords
  • Evolutionary genomics
  • Gene regulation
  • Gonad development
  • Moles
  • Ovotestes
  • SALL1
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Funding
Citation (ISO format)
SCHINDLER, Magdalena et al. Induction of kidney-related gene programs through co-option of <i>SALL1</i> in mole ovotestes. In: Development, 2023, vol. 150, n° 17, p. dev201562. doi: 10.1242/dev.201562
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ISSN of the journal0950-1991
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