en
Scientific article
Open access
English

CD1d-restricted T cells regulate dendritic cell function and antitumor immunity in a granulocyte-macrophage colony-stimulating factor-dependent fashion

Publication date2003-07-22
First online date2003-07-07
Abstract

CD1d-restricted T cells contribute to tumor protection, but their precise roles remain unclear. Here we show that tumor cells engineered to secrete granulocyte-macrophage colony-stimulating factor induce the expansion of CD1d-restricted T cells through a mechanism that involves CD1d and macrophage inflammatory protein 2 expression by CD8 alpha-, CD11c+ dendritic cells (DCs). The antitumor immunity stimulated by vaccination with irradiated, granulocyte-macrophage colony-stimulating factor-secreting tumor cells was abrogated in CD1d- and J alpha 281-deficient mice, revealing a critical role for CD1d-restricted T cells in this response. The loss of antitumor immunity was associated with impaired tumor-induced T helper 2 cytokine production, although IFN-gamma secretion and cytotoxicity were preserved. DCs from immunized CD1d-deficient mice showed compromised maturation and function. Together, these results delineate a role for CD1d-restricted T cell-DC cross talk in the shaping of antitumor immunity.

eng
Keywords
  • Animals
  • Antigens, CD1 / genetics
  • Antigens, CD1 / metabolism
  • Antigens, CD1d
  • Cancer Vaccines / immunology
  • Cancer Vaccines / pharmacology
  • Chemokine CXCL2
  • Chemokines / genetics
  • Dendritic Cells / immunology
  • Female
  • Gene Expression
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
  • Immunity, Cellular
  • In Vitro Techniques
  • Killer Cells, Natural / immunology
  • Melanoma, Experimental / immunology
  • Melanoma, Experimental / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • T-Lymphocyte Subsets / drug effects
  • T-Lymphocyte Subsets / immunology
Affiliation Not a UNIGE publication
Citation (ISO format)
GILLESSEN, Silke et al. CD1d-restricted T cells regulate dendritic cell function and antitumor immunity in a granulocyte-macrophage colony-stimulating factor-dependent fashion. In: Proceedings of the National Academy of Sciences of the United States of America, 2003, vol. 100, n° 15, p. 8874–8879. doi: 10.1073/pnas.1033098100
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0027-8424
11views
3downloads

Technical informations

Creation12/06/2023 2:53:51 PM
First validation03/20/2024 10:45:36 AM
Update time03/20/2024 10:45:36 AM
Status update03/20/2024 10:45:36 AM
Last indexation05/06/2024 6:13:28 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack