Scientific article
Open access

CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity

Published inThe Journal of cell biology, vol. 222, no. 12, e202305009
Publication date2023-12-04
First online date2023-11-07

Centrioles are microtubule-based organelles responsible for forming centrosomes and cilia, which serve as microtubule-organizing, signaling, and motility centers. Biogenesis and maintenance of centrioles with proper number, size, and architecture are vital for their functions during development and physiology. While centriole number control has been well-studied, less is understood about their maintenance as stable structures with conserved size and architecture during cell division and ciliary motility. Here, we identified CCDC15 as a centriole protein that colocalizes with and interacts with the inner scaffold, a crucial centriolar subcompartment for centriole size control and integrity. Using ultrastructure expansion microscopy, we found that CCDC15 depletion affects centriole length and integrity, leading to defective cilium formation, maintenance, and response to Hedgehog signaling. Moreover, loss-of-function experiments showed CCDC15’s role in recruiting both the inner scaffold protein POC1B and the distal SFI1/Centrin-2 complex to centrioles. Our findings reveal players and mechanisms of centriole architectural integrity and insights into diseases linked to centriolar defects.

  • European Commission - Dissecting the function and regulation of centriolar satellites: key regulators of the centrosome/cilium complex [679140]
Citation (ISO format)
ARSLANHAN, Melis D. et al. CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity. In: The Journal of cell biology, 2023, vol. 222, n° 12, p. e202305009. doi: 10.1083/jcb.202305009
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Article (Published version)
ISSN of the journal0021-9525

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