en
Scientific article
English

A highly soluble Sleeping Beauty transposase improves control of gene insertion

Published inNature biotechnology, vol. 37, no. 12, p. 1502-1512
Publication date2019-11-04
First online date2019-11-04
Abstract

The Sleeping Beauty (SB) transposon system is an efficient non-viral gene transfer tool in mammalian cells, but its broad use has been hampered by uncontrolled transposase gene activity from DNA vectors, posing a risk of genome instability, and by the inability to use the transposase protein directly. In this study, we used rational protein design based on the crystal structure of the hyperactive SB100X variant to create an SB transposase (high-solubility SB, hsSB) with enhanced solubility and stability. We demonstrate that hsSB can be delivered with transposon DNA to genetically modify cell lines and embryonic, hematopoietic and induced pluripotent stem cells (iPSCs), overcoming uncontrolled transposase activity. We used hsSB to generate chimeric antigen receptor (CAR) T cells, which exhibit potent antitumor activity in vitro and in xenograft mice. We found that hsSB spontaneously penetrates cells, enabling modification of iPSCs and generation of CAR T cells without the use of transfection reagents. Titration of hsSB to modulate genomic integration frequency achieved as few as two integrations per genome.

eng
Affiliation Not a UNIGE publication
Funding
  • Deutsche Krebshilfe - [Max Eder Program Award 110313]
  • Paul Ehrlich Institute -
  • Bayerische Akademie der Wissenschaften - [m4 Award in Personalized Medicine]
  • Hessisches Ministerium für Wissenschaft und Kunst - [LOEWE]
  • EMBL - [EMBL001]
  • EMBL International PhD Programme -
  • European Molecular Biology Laboratory -
  • Versus Arthritis - [EMBL_EMBL001]
Citation (ISO format)
QUERQUES, Irma et al. A highly soluble Sleeping Beauty transposase improves control of gene insertion. In: Nature biotechnology, 2019, vol. 37, n° 12, p. 1502–1512. doi: 10.1038/s41587-019-0291-z
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1087-0156
22views
0downloads

Technical informations

Creation03/10/2024 9:24:32 AM
First validation03/11/2024 10:42:10 AM
Update time03/11/2024 10:42:10 AM
Status update03/11/2024 10:42:10 AM
Last indexation05/06/2024 6:08:27 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack