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The Alu domain homolog of the yeast signal recognition particle consists of an Srp14p homodimer and a yeast-specific RNA structure

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Published in RNA. 1999, vol. 5, no. 10, p. 1333-47
Abstract The mammalian Alu domain of the signal recognition particle (SRP) consists of a heterodimeric protein SRP9/14 and the Alu portion of 7SL RNA and comprises the elongation arrest function of the particle. To define the domain in Saccharomyces cerevisiae SRP that is homologous to the mammalian Alu domain [Alu domain homolog in yeast (Adhy)], we examined the assembly of a yeast protein homologous to mammalian SRP14 (Srp14p) and scR1 RNA. Srp14p binds as a homodimeric complex to the 5' sequences of scR1 RNA. Its minimal binding site consists of 99 nt. (Adhy RNA), comprising a short hairpin structure followed by an extended stem. As in mammalian SRP9/14, the motif UGUAAU present in most SRP RNAs is part of the Srp14p binding sites as shown by footprint and mutagenesis studies. In addition, certain basic amino acid residues conserved between mammalian SRP14 and Srp14p are essential for RNA binding in both proteins. These findings confirm the common ancestry of the yeast and the mammalian components and indicate that Srp14p together with Adhy RNA represents the Alu domain homolog in yeast SRP that may comprise its elongation arrest function. Despite the similarities, Srp14p selectively recognizes only scR1 RNA, revealing substantial changes in RNA-protein recognition as well as in the overall structure of the complex. The alignment of the three yeast SRP RNAs known to date suggests a common structure for the putative elongation arrest domain of all three organisms.
Keywords Alu ElementsAmino Acid SequenceBase SequenceConserved SequenceDNA, FungalDimerizationEvolution, MolecularMolecular Sequence DataNucleic Acid ConformationRNA, Fungal/chemistry/metabolismRNA-Binding Proteins/metabolismSaccharomyces cerevisiae/geneticsSaccharomyces cerevisiae ProteinsSignal Recognition Particle/genetics/metabolism
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PMID: 10573124
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STRUB, Katharina, FORNALLAZ-MULHAUSER, Monique, BUI, Nazarena. The Alu domain homolog of the yeast signal recognition particle consists of an Srp14p homodimer and a yeast-specific RNA structure. In: RNA, 1999, vol. 5, n° 10, p. 1333-47. https://archive-ouverte.unige.ch/unige:17523

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Deposited on : 2011-11-24

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