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Scientific article
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English

Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice

Publication date2013-09-25
First online date2013-09-25
Abstract

Large parts of eukaryotic genomes are composed of transposons. Mammalian genomes use DNA methylation to silence these genomic parasites. A class of small RNAs called Piwi-interacting RNAs (piRNAs) is used to specifically guide the DNA methylation machinery to the transposon DNA elements. How germ cells make piRNAs is not entirely understood. We identify a mouse protein and demonstrate its importance for transposon silencing. We find that the protein collaborates with other factors already implicated in piRNA production. Moreover, the protein is required for piRNA production and assembly of the nuclear silencing complex. Physiological importance of the protein is highlighted by the fact that male mice lacking the protein are infertile. This study will greatly benefit the field of germ-cell biology.

eng
Keywords
  • DNA methylation
  • Helicase
  • Spermatogenesis
Affiliation Not a UNIGE publication
Funding
  • NHGRI NIH HHS - [R21 HG007394]
Citation (ISO format)
PANDEY, Radha Raman et al. Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice. In: Proceedings of the National Academy of Sciences of the United States of America, 2013, vol. 110, n° 41, p. 16492–16497. doi: 10.1073/pnas.1316316110
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Article (Published version)
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Identifiers
ISSN of the journal0027-8424
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