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Scientific article
English

Microscopy of Fission Yeast Sexual Lifecycle

Published inJournal of visualized experiments, no. 109
Publication date2016-03-09
First online date2016-03-09
Abstract

The fission yeast Schizosaccharomyces pombe has been an invaluable model system in studying the regulation of the mitotic cell cycle progression, the mechanics of cell division and cell polarity. Furthermore, classical experiments on its sexual reproduction have yielded results pivotal to current understanding of DNA recombination and meiosis. More recent analysis of fission yeast mating has raised interesting questions on extrinsic stimuli response mechanisms, polarized cell growth and cell-cell fusion. To study these topics in detail we have developed a simple protocol for microscopy of the entire sexual lifecycle. The method described here is easily adjusted to study specific mating stages. Briefly, after being grown to exponential phase in a nitrogen-rich medium, cell cultures are shifted to a nitrogen-deprived medium for periods of time suited to the stage of the sexual lifecycle that will be explored. Cells are then mounted on custom, easily built agarose pad chambers for imaging. This approach allows cells to be monitored from the onset of mating to the final formation of spores.

eng
Keywords
  • Molecular Biology
  • Issue 109
  • Fission yeast
  • Schizosaccharomyces pombe
  • Live-cell imaging
  • Fluorescence microscopy
  • Nitrogen starvation
  • Pheromone
  • G protein-coupled receptor,
  • Sex
  • Mating
  • Shmoo
  • Cell-cell fusion
Affiliation Not a UNIGE publication
Citation (ISO format)
VJESTICA, Aleksandar et al. Microscopy of Fission Yeast Sexual Lifecycle. In: Journal of visualized experiments, 2016, n° 109. doi: 10.3791/53801
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Article (Published version)
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Identifiers
ISSN of the journal1940-087X
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