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Scientific article
English

Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails

Published inScience, vol. 363, no. 6424, p. 285-288
Publication date2019-01-18
Abstract

Microtubule doublets (MTDs), consisting of an incomplete B-microtubule at the surface of a complete A-microtubule, provide a structural scaffold mediating intraflagellar transport and ciliary beating. Despite the fundamental role of MTDs, the molecular mechanism governing their formation is unknown.We used a cell-free assay to demonstrate a crucial inhibitory role of the carboxyl-terminal (C-terminal) tail of tubulin in MTD assembly. Removal of the C-terminal tail of an assembled A-microtubule allowed for the nucleation of a B-microtubule on its surface. C-terminal tails of only one A-microtubule protofilament inhibited this side-to-surface tubulin interaction, which would be overcome in vivo with binding protein partners. The dynamics of B-microtubule nucleation and its distinctive isotropic elongation was elucidated by using live imaging. Thus, inherent interaction properties of tubulin provide a structural basis driving flagellar MTD assembly.

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Funding
  • European Commission - Unravelling the architecture and the cartography of the human centriole [715289]
Citation (ISO format)
CERNOHORSKA, Marketa et al. Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. In: Science, 2019, vol. 363, n° 6424, p. 285–288. doi: 10.1126/science.aav2567
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ISSN of the journal0036-8075
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