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Scientific article
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SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture

Published inNature cell biology, vol. 18, no. 4, p. 393-403
Publication date2016-03-21
First online date2016-03-21
Abstract

Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS-6-based oligomers with symmetries ranging from five- to ten-fold. Expression of a SAS-6 mutant that forms six-fold symmetric cartwheel structures in vitro resulted in cartwheels and centrioles with eight- or nine-fold symmetries in vivo. In combination with Bld10 mutants that weaken cartwheel-microtubule interactions, this SAS-6 mutant produced six- to eight-fold symmetric cartwheels. Concurrently, the microtubule wall maintained eight- and nine-fold symmetries. Expressing SAS-6 with analogous mutations in human cells resulted in nine-fold symmetric centrioles that exhibited impaired length and organization. Together, our data suggest that the self-assembly properties of SAS-6 instruct cartwheel symmetry, and lead us to propose a model in which the cartwheel and the microtubule wall assemble in an interdependent manner to establish the native architecture of centrioles.

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Affiliation Not a UNIGE publication
Citation (ISO format)
HILBERT, Manuel et al. SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. In: Nature cell biology, 2016, vol. 18, n° 4, p. 393–403. doi: 10.1038/ncb3329
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ISSN of the journal1465-7392
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