Characterization of APOBEC3G binding to 7SL RNA

Bach, Daniel; Peddi, Shyam; Mangeat, Bastien; Lakkaraju, Asvin Krishna Kumar; Strub, Katharina; Trono, Didier

doi:10.1186/1742-4690-5-54

Scientific article

English

Characterization of APOBEC3G binding to 7SL RNA

Contributors

Bach, Daniel; Peddi, Shyam; Mangeat, Bastien; Lakkaraju, Asvin Krishna Kumar; Strub, Katharina; Trono, Didier

Published in

Retrovirology, vol. 5, 54

Publication date

2008

Abstract

Human APOBEC3 proteins are editing enzymes that can interfere with the replication of exogenous retroviruses such as human immunodeficiency virus (HIV), hepadnaviruses such as hepatitis B virus (HBV), and with the retrotransposition of endogenous retroelements such as long-interspersed nuclear elements (LINE) and Alu. Here, we show that APOBEC3G, but not other APOBEC3 family members, binds 7SL RNA, the common ancestor of Alu RNAs that is specifically recruited into HIV virions. Our data further indicate that APOBEC3G recognizes 7SL RNA and Alu RNA by its common structure, the Alu domain, suggesting a mechanism for APOBEC3G- mediated inhibition of Alu retrotransposition. However, we also demonstrate that APOBEC3F and APOBEC3G are normally recruited into and inhibit the infectivity of DeltaVif HIV1 virions when 7SLRNA is prevented from accessing particles by RNA interference against SRP14 or by over expression of SRP19, both components of the signal recognition particle. We thus conclude that 7SL RNA is not an essential mediator of the virion packaging of these antiviral cytidine deaminases.

Keywords

Alu Elements/genetics/physiology
Base Sequence
Cell Line
Cytidine Deaminase/genetics/metabolism
HIV-1/metabolism
Humans
Molecular Sequence Data
Mutation
RNA, Small Cytoplasmic/chemistry/metabolism
Signal Recognition Particle/chemistry/metabolism
Virion/metabolism
Virus Assembly

UNIGE affiliation entities

Faculté des sciences / Section de biologie / Département de biologie cellulaire

Citation (ISO format)

BACH, Daniel et al. Characterization of APOBEC3G binding to 7SL RNA. In: Retrovirology, 2008, vol. 5, p. 54. doi: 10.1186/1742-4690-5-54

Article (Published version)

Identifiers

PID : unige:17488
DOI : 10.1186/1742-4690-5-54
PMID : 18597676

Journal ISSN

1742-4690

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Creation

17/11/2011 17:46:00

First validation

17/11/2011 17:46:00

Update

14/03/2023 17:04:30

Status update

14/03/2023 17:04:30

Last indexation

29/10/2024 18:36:24

Characterization of APOBEC3G binding to 7SL RNA

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation