An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells

Castelnuovo, Manuele; Massone, Sara; Tasso, Roberta; Fiorino, Gloria; Gatti, Monica; Robello, Mauro; Gatta, Elena; Berger, Audrey; Strub, Katharina; Florio, Tullio; Dieci, Giorgio; Cancedda, Ranieri; Pagano, Aldo

doi:10.1096/fj.10-157032

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Title		An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells
Authors		Castelnuovo, Manuele Massone, Sara Tasso, Roberta Fiorino, Gloria Gatti, Monica Robello, Mauro Gatta, Elena Berger, Audrey Strub, Katharina Florio, Tullio Dieci, Giorgio Cancedda, Ranieri Pagano, Aldo show all authors [1 - 13]
Published in		The FASEB journal. 2010, vol. 24, no. 10, p. 4033-46
Abstract		Neuroblastoma (NB) is a pediatric cancer characterized by remarkable cell heterogeneity within the tumor nodules. Here, we demonstrate that the synthesis of a pol III-transcribed noncoding (nc) RNA (NDM29) strongly restricts NB development by promoting cell differentiation, a drop of malignancy processes, and a dramatic reduction of the tumor initiating cell (TIC) fraction in the NB cell population. Notably, the overexpression of NDM29 also confers to malignant NB cells an unpredicted susceptibility to the effects of antiblastic drugs used in NB therapy. Altogether, these results suggest the induction of NDM29 expression as possible treatment to increase cancer cells vulnerability to therapeutics and the measure of its synthesis in NB explants as prognostic factor of this cancer type.
Keywords		Alu Elements — Base Sequence — Cell Adhesion — Cell Cycle — Cell Differentiation/genetics — DNA Primers — Down-Regulation — Fluorescent Antibody Technique — Humans — Neuroblastoma/pathology — Tumor Cells, Cultured
Identifiers		DOI: 10.1096/fj.10-157032 PMID: 20581224
Full text		Article (Published version) (7.6 MB) - Free access
Structures		Faculté des sciences / Section de biologie / Département de biologie cellulaire
Research group		Groupe Strub
Citation (ISO format)		CASTELNUOVO, Manuele et al. An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells. In: The FASEB journal, 2010, vol. 24, n° 10, p. 4033-46. doi: 10.1096/fj.10-157032 https://archive-ouverte.unige.ch/unige:17485