Scientific article
OA Policy
English

DNA polymerases ν and θ are required for efficient immunoglobulin V gene diversification in chicken

Published inThe journal of cell biology, vol. 189, no. 7, p. 1117-1127
Publication date2010-06-28
First online date2010-06-28
Abstract

The chicken DT40 B lymphocyte line diversifies its immunoglobulin (Ig) V genes through translesion DNA synthesis–dependent point mutations (Ig hypermutation) and homologous recombination (HR)–dependent Ig gene conversion. The error-prone biochemical characteristic of the A family DNA polymerases Polν and Polθ led us to explore the role of these polymerases in Ig gene diversification in DT40 cells. Disruption of both polymerases causes a significant decrease in Ig gene conversion events, although POLN−/−/POLQ−/− cells exhibit no prominent defect in HR-mediated DNA repair, as indicated by no increase in sensitivity to camptothecin. Polη has also been previously implicated in Ig gene conversion. We show that a POLH−/−/POLN−/−/POLQ−/− triple mutant displays no Ig gene conversion and reduced Ig hypermutation. Together, these data define a role for Polν and Polθ in recombination and suggest that the DNA synthesis associated with Ig gene conversion is accounted for by three specialized DNA polymerases.

Research groups
Citation (ISO format)
KOZAKI, Masaoki et al. DNA polymerases ν and θ are required for efficient immunoglobulin V gene diversification in chicken. In: The journal of cell biology, 2010, vol. 189, n° 7, p. 1117–1127. doi: 10.1083/jcb.200912012
Main files (1)
Article (Published version)
Identifiers
Journal ISSN0021-9525
31views
7downloads

Technical informations

Creation03/01/2024 10:01:29
First validation07/02/2024 11:41:12
Update time07/02/2024 11:41:12
Status update07/02/2024 11:41:12
Last indexation01/11/2024 08:26:52
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack