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Scientific article
Open access
English

gem‐Diethyl Pyrroline Nitroxide Spin Labels: Synthesis, EPR Characterization, Rotamer Libraries and Biocompatibility

Published inChemistryOpen, vol. 8, no. 8, p. 1057-1065
Publication date2019-05-14
First online date2019-05-14
Abstract

The availability of bioresistant spin labels is crucial for the optimization of site‐directed spin labeling protocols for EPR structural studies of biomolecules in a cellular context. As labeling can affect proteins’ fold and/or function, having the possibility to choose between different spin labels will increase the probability to produce spin‐labeled functional proteins. Here, we report the synthesis and characterization of iodoacetamide‐ and maleimide‐functionalized spin labels based on the gem‐diethyl pyrroline structure. The two nitroxide labels are compared to conventional gem‐dimethyl analogs by site‐directed spin labeling (SDSL) electron paramagnetic resonance (EPR) spectroscopy, using two water soluble proteins: T4 lysozyme and Bid. To foster their use for structural studies, we also present rotamer libraries for these labels, compatible with the MMM software. Finally, we investigate the “true” biocompatibility of the gem ‐diethyl probes comparing the resistance towards chemical reduction of the NO group in ascorbate solutions and E. coli cytosol at different spin concentrations.

eng
Keywords
  • EPR
  • Biocompatibility
  • Nitroxide
  • Pyrrolines
  • Spin labeling
Affiliation Not a UNIGE publication
Funding
  • National Institutes of Health - [NIGMS R01 GM124310-01]
  • NIGMS NIH HHS - [R01 GM124310]
  • National Science Foundation - [NSF/CHE-1346572]
Citation (ISO format)
BLEICKEN, Stephanie et al. <i>gem</i>‐Diethyl Pyrroline Nitroxide Spin Labels: Synthesis, EPR Characterization, Rotamer Libraries and Biocompatibility. In: ChemistryOpen, 2019, vol. 8, n° 8, p. 1057–1065. doi: 10.1002/open.201900119
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Article (Published version)
Identifiers
ISSN of the journal2191-1363
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