Scientific article
Open access

Low density membranes are associated with RNA-binding proteins and thylakoids in the chloroplast of Chlamydomonas reinhardtii

Published inThe Journal of cell biology, vol. 140, no. 1, p. 101-110
Publication date1998-01-12

Chloroplast subfractions were tested with a UV cross-linking assay for proteins that bind to the 5' untranslated region of the chloroplast psbC mRNA of the green alga Chlamydomonas reinhardtii. These analyses revealed that RNA-binding proteins of 30-32, 46, 47, 60, and 80 kD are associated with chloroplast membranes. The buoyant density and the acyl lipid composition of these membranes are compatible with their origin being the inner chloroplast envelope membrane. However, unlike previously characterized inner envelope membranes, these membranes are associated with thylakoids. One of the membrane-associated RNA-binding proteins appears to be RB47, which has been reported to be a specific activator of psbA mRNA translation. These results suggest that translation of chloroplast mRNAs encoding thylakoid proteins occurs at either a subfraction of the chloroplast inner envelope membrane or a previously uncharacterized intra-chloroplast compartment, which is physically associated with thylakoids.

  • Animals
  • Cell Fractionation
  • Chlamydomonas reinhardtii / metabolism
  • Chloroplasts / metabolism
  • Chloroplasts / ultrastructure
  • Intracellular Membranes / metabolism
  • Intracellular Membranes / ultrastructure
  • Molecular Weight
  • Organelles / metabolism
  • Organelles / ultrastructure
  • Photosynthetic Reaction Center Complex Proteins / biosynthesis
  • Photosystem II Protein Complex
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / isolation & purification
  • RNA-Binding Proteins / metabolism
Citation (ISO format)
ZERGES, William, ROCHAIX, Jean-David. Low density membranes are associated with RNA-binding proteins and thylakoids in the chloroplast of <i>Chlamydomonas reinhardtii</i>. In: The Journal of cell biology, 1998, vol. 140, n° 1, p. 101–110. doi: 10.1083/jcb.140.1.101
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Article (Published version)
ISSN of the journal0021-9525

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