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Scientific article
Open access
English

Contrasting behavior between the three human monocyte subsets in dengue pathophysiology

Published iniScience, vol. 25, no. 6, 104384
Publication date2022-06-17
First online date2022-05-10
Abstract

Monocytes are known to play a critical role in dengue pathophysiology. However, which monocyte subset expresses what inflammatory mediator(s) and what transcriptional features distinguish each of the monocyte subsetin vivoremain poorly understood. In this study we provide a detailed transcriptional analysis of the three human monocyte subsets in healthy children and in children with dengue febrile illness. Notably, we found that the CD14+CD16high intermediate monocyte subset from dengue patients highly upregulated key genes involved in mediating inflammation, endothelial dysfunction, vascular permeability, tissue extravasation, and clot prevention compared to healthy children. The CD14+CD16low classical monocytes shared some of these features. These two subsets increased massively in patients with severe dengue. By contrast, the CD14-CD16high nonclassical monocyte subset upregulated key genes involved in vasoconstriction, endothelial barrier stability, and are involved in endothelial patrolling while showing a significant decline from circulation. These findings improve our understanding of monocyte responses in dengue.

eng
Keywords
  • Immunology
  • Omics
  • Virology
Affiliation Not a UNIGE publication
Citation (ISO format)
MAHESHWARI, Deepti et al. Contrasting behavior between the three human monocyte subsets in dengue pathophysiology. In: iScience, 2022, vol. 25, n° 6, p. 104384. doi: 10.1016/j.isci.2022.104384
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ISSN of the journal2589-0042
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