UNIGE document Scientific Article - Meta-analysis
previous document  unige:17358  next document
add to browser collection

The mouse interleukin (Il)33 gene is expressed in a cell type- and stimulus-dependent manner from two alternative promoters

Published in Journal of leukocyte biology. 2012, vol. 91, no. 1, p. 119-125
Abstract GenBank entries for mouse Il33 reveal the existence of two transcripts, Il33a and Il33b, with different 5`UTRs but coding for the same protein. We investigated expression of these transcripts in different mouse organs and cell types in basal and inflammatory conditions. Il33a and Il33b mRNAs start with different noncoding first exons, transcribed from different promoter regions, which both contain a consensus TATA-like sequence. Constitutive Il33a mRNA expression was detected in mouse stomach, lung, spleen, and brain, whereas basal Il33b mRNA expression was observed only in the stomach. Expression of both transcripts increased after systemic LPS administration. In vitro, we observed high constitutive expression of Il33 transcripts in MEFs. Constitutive Il33a mRNA expression was observed also in BMDCs, where it was preferentially increased in response to poly(I:C), whereas LPS increased levels of Il33a and Il33b mRNA. In contrast, BMMs and Raw 264.7 cells did not express Il33 mRNA constitutively, and LPS stimulation selectively induced expression of Il33b mRNA in these cells. Our data indicate that the Il33 gene is expressed from two alternative promoters in the mouse and that the relative expression of Il33a and Il33b transcripts is cell type- and stimulus-dependent.
PMID: 22013230
Full text
Article (Accepted version) (778 Kb) - private document Private access
Research groups Biologie des cytokines de la famille de l'interleukine-1 (1022)
Mécanisme de l'inflammation articulaire (44)
(ISO format)
TALABOT FRISCHKNECHT-AYER, Dominique et al. The mouse interleukin (Il)33 gene is expressed in a cell type- and stimulus-dependent manner from two alternative promoters. In: Journal of leukocyte biology, 2012, vol. 91, n° 1, p. 119-125. doi: 10.1189/jlb.0811425 https://archive-ouverte.unige.ch/unige:17358

506 hits

1 download


Deposited on : 2011-11-09

Export document
Format :
Citation style :