en
Scientific article
Open access
English

Myosin heavy-chain messenger ribonucleic acid (mRNA) expression and fibre cross-sectional area in masseter, digastric, gastrocnemius and soleus muscles of young and adult rats

Published inBiology, vol. 12, no. 6, 842
Publication date2023-06-09
First online date2023-06-09
Abstract

Different demands on the muscles of mastication may influence their functional profile (size and distribution of muscle fibre types), which may change during growth and maturation, potentially influencing craniofacial growth. The aim of this study was to evaluate mRNA expression and cross-sectional area of masticatory muscle fibres compared with limb muscles in young and adult rats. Twenty-four rats were sacrificed at two different ages, namely 12 at 4 weeks (young) and 12 at 26 weeks (adult). The masseter, digastric, gastrocnemius and soleus muscles were dissected. Gene expression of myosin heavy-chain isoformsMyh7(MyHC-I),Myh2(MyHC-IIa),Myh4(MyHC-IIb) andMyh1(MyHC-IIx) in the muscles was measured using qRT-PCR RNA analysis, and immunofluorescence staining was performed to measure the cross-sectional area of different muscle fibre types. Different muscle types and ages were compared. Significant differences were found in the functional profile between masticatory and limb muscles. For the masticatory muscles, there was an increase inMyh4expression with age, and this change was more intense for the masseter muscles, which also presented an increase inMyh1expression, similarly to limb muscles. The fibre cross-sectional area of the masticatory muscles was generally smaller in young rats; however, this difference was less pronounced than in limb muscles.

eng
Keywords
  • Diet
  • Masticatory function
  • Masticatory muscles
Citation (ISO format)
LAGOU, Aikaterini et al. Myosin heavy-chain messenger ribonucleic acid (mRNA) expression and fibre cross-sectional area in masseter, digastric, gastrocnemius and soleus muscles of young and adult rats. In: Biology, 2023, vol. 12, n° 6, p. 842. doi: 10.3390/biology12060842
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal2079-7737
28views
4downloads

Technical informations

Creation11/10/2023 1:54:33 PM
First validation11/13/2023 9:36:29 AM
Update time11/13/2023 9:36:29 AM
Status update11/13/2023 9:36:29 AM
Last indexation05/06/2024 5:19:39 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack