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High-yield production of valepotriates by hairy root cultures of Valeriana officinalis L. var. sambucifolia Mikan

Published inPlant cell reports, vol. 11, p. 339-342
Publication date1992
Abstract

Hairy root cultures of Valeriana officinalis var. sambucifolia were established by infection of sterile plantlets with Agrobacterium rhizogenes strain R1601.The transformed roots were grown in 10 different, hormone-free liquid media and the isovaltrate, valtrate, didrovaltrate, isovaleroxyhydroxydidrovaltrate content was quantified by HPLC. Valepotriates were entirely retained inside the root tissues. The highest overall valepotriate content (10.3 % dry wt), 4 times the amount found in the roots of 9-month-old nontransformed plants, was observed in half strength Gamborg B5 medium supplemented with 2 % sucrose. The hairy roots cultured in Murashige and Skoog liquid medium supplemented with 2 % sucrose for 50 days produced over 44 mg/g dry wt valepotriates.

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Keywords
  • Sucrose
  • Liquid Medium
  • Hairy Root
  • Root Tissue
  • Root Culture
Citation (ISO format)
GRAENICHER, François Philippe, CHRISTEN, Philippe, KAPETANIDIS, Ilias. High-yield production of valepotriates by hairy root cultures of <i>Valeriana officinalis </i>L. var. <i>sambucifolia</i> Mikan. In: Plant cell reports, 1992, vol. 11, p. 339–342. doi: 10.1007/BF00233361
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ISSN of the journal0721-7714
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