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Production of diosgenin by hairy root cultures of Trigonella foenum-graecum L

Published inPlant cell reports, vol. 16, no. 9, p. 632-636
Publication date1997-06
Abstract

Hairy root cultures of Trigonella foenum-graecum L. were established with Agrobacterium rhizogenes strain A4. The hairy roots produce diosgenin, an important spirostanol for the semi-synthesis of steroid hormones. Fourteen different liquid media were investigated. The fastest growth was obtained in McCown’s woody plant (WP) medium supplemented with 3% sucrose; the highest diosgenin content was observed in half-strength WP medium with 1% sucrose (0.040% dry weight), which represents almost twice the amount detected in the 8-month-old non-transformed roots (0.024%). A time-course study in WP liquid media supplemented with 3% sucrose was undertaken. In these conditions, 17 µg diosgenin/g fresh weight were produced. The influence of cholesterol, medium pH and chitosan on diosgenin production was tested. The addition of 40 mg/l chitosan elevated the diosgenin content to three times that found in non-elicited hairy roots.

eng
Keywords
  • Elicitor
  • Hairy roots Diosgenin
  • Trigonella foenum-graecum
Citation (ISO format)
MERKLI, Alain, CHRISTEN, Philippe, KAPETANIDIS, Ilias. Production of diosgenin by hairy root cultures of <i>Trigonella foenum-graecum</i> L. In: Plant cell reports, 1997, vol. 16, n° 9, p. 632–636. doi: 10.1007/BF01275505
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ISSN of the journal0721-7714
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