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Scientific article
English

α₅ subunit forms functional α₃β₄α₅ nAChRs in transfected human cells

Published inNeuroreport, vol. 8, no. 11, p. 2433-2436
Publication date1997-07-28
Abstract

NAChRs heterologously expressid in human cells after transient transfection with α₃β₄α₅ or α₃β₄ subunit cDNAs exhibited similar sensitivities to antogonists and comparable functional channel profiles. However, the sum of two Hill equations was required for best fitting the ACh dose-current response curves after co-expression of α₅ α₃ and β₄ subunits. One component was comparable to that obtained in α₃β₄-transfected cells, while the additional component, putatively attributed to an α₃β₄α₅ nAChR population, showed a Hill coeeficient > 2 and a nine-fold greater half-maximal ACh concentration (EC50). These results suggest that the α₅ subunit participates in the assembly of α₃β₄α₅ nAChRs compelxes in human cells, adding a new member to the family of neuronal nicotinic receptors.

eng
Keywords
  • Cell transfection
  • Nicotinic acetylcholine receptor
  • Oocytes
  • Patch clamp
Citation (ISO format)
FUCILE, Sergio et al. α₅ subunit forms functional α₃β₄α₅ nAChRs in transfected human cells. In: Neuroreport, 1997, vol. 8, n° 11, p. 2433–2436.
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Article (Published version)
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Identifiers
  • PID : unige:172077
ISSN of the journal0959-4965
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