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Scientific article
Open access
English

Detection of genomic mutations in blood and urine free circulating tumour DNA in patients with inoperable and metastatic lung adenocarcinoma harbouring an EGFR mutation in tissue: a UK pilot study

Published inFrontiers in oncology, vol. 13, 1197037
Publication date2023
First online date2023-07-05
Abstract

The development of methodologies to analyse circulating tumour DNA (ctDNA) in the blood or urine of cancer patients provides an invaluable resource that can be used for diagnosis and prognosis and to evaluate response to treatments. Lung cancer has seen in the last years a revolution in treatment strategy with the use of several classes of EGFR inhibitors. However, almost invariably, resistance to such therapies appears. In this paper, we describe a pilot, longitudinal study with 20 patients with confirmedEGFRmutations in tissue biopsy for lung cancer. The objective of the study was to determine whether ctDNA from plasma and/or urine could be used to monitor theEGFRmutational status of patients with confirmedEGFRmutation-positive non-small cell lung cancer (NSCLC) during treatment with EGFR inhibitors. Blood and urine were collected monthly over periods ranging from 6 to 16 months. CtDNA was analysed in each patient for the presence of several known mutations that predispose to resistance to EGFR inhibitors. We have proven that serial monitoring of ctDNA from both plasma and urine is feasible and that patients are willing to participate in this process. We have also shown that longitudinal ctDNA monitoring may detect resistance mutations before the development of radiological and clinical disease progression.

eng
Keywords
  • CtDNA
  • EGFR
  • Lung cancer
  • Plasma
  • Resistance to TKIs
  • Urine
Citation (ISO format)
BROOKS, Helen et al. Detection of genomic mutations in blood and urine free circulating tumour DNA in patients with inoperable and metastatic lung adenocarcinoma harbouring an EGFR mutation in tissue: a UK pilot study. In: Frontiers in oncology, 2023, vol. 13, p. 1197037. doi: 10.3389/fonc.2023.1197037
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ISSN of the journal2234-943X
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Creation07/31/2023 12:42:10 PM
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