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Evaluation of the nodulated status of Vigna unguiculata probed by the JIP-test based on the chlorophyll α fluorescence rise

Published inPS2001 proceedings, p. S36-012
Presented at 12th International Congress on Photosynthesis, Brisbane, 2001
PublisherCollingwood : CSIRO Publishing
Publication date2001
Abstract

Chlorophyll α fluorescence is a useful and non-invasive tool to screen for the effects of many biotic and abiotic parameters on photosynthesis in plants. The fluorescence of leaves after excitation with light was measured with a portable fluorimeter (Plant Efficiency Analyser, built by Hansatech Instruments King's Lynn Norfolk, PE 30 4 NE, GB) and the subsequent data were analysed using the JIP-test which provides parameters indicating PSII properties. Seeds of Vigna unguiculata were sterilized before germinating and planting in Magenta jars (used in hydroponics culture) with a nutrient solution without nitrate (B&D solution). Some of the plants were inoculated with Rhizobium sp. strain NGR234 (1019 bacteria/100μl) at roots level. The other plants were treated with different KN03 concentrations (0 mM, 0.5 mM, 1 mM, 5 mM, 10 mM and 20 mM) to produce several treatments including that with Rhizobium. During 6 weeks several hundred fluorescence measurements of 2 seconds are made on the leaves of inoculated and control plants, with or without different nitrate (KN03) concentrations. The results obtained suggest that this technique can screen nodulation as well as nitrogen deficiency in vivo on the level of the leaves. The results of KN03 and the influence of nodulation and KN03 on primary photosynthesis will be discussed in respect to the modifications observed in the fast fluorescence rise O-K-J-I-P.

eng
Keywords
  • Fluorescence
  • OJIP-rise
  • Rhizobium sp. strain NGR234
  • Vigna unguiculata
Citation (ISO format)
SCHMITZ, P., MALDONADO RODRIGUEZ, Ronald, STRASSER, Reto. Evaluation of the nodulated status of <i>Vigna unguiculata</i> probed by the JIP-test based on the chlorophyll <i>α</i> fluorescence rise. In: PS2001 proceedings. Brisbane. Collingwood : CSIRO Publishing, 2001. p. S36–012.
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Proceedings chapter (Published version)
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  • PID : unige:171805
ISBN0643067116
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