Surface-sterilization of <i>Glomus mosseae</i> sporocarps for studying endomycorrhization in vitro

Budi, Sri Wilarso; Blal, Bachar; Gianinazzi, Silvio

doi:10.1007/s005720050001

Scientific article

English

Surface-sterilization of Glomus mosseae sporocarps for studying endomycorrhization in vitro

ContributorsBudi, Sri Wilarso; Blal, Bachar; Gianinazzi, Silvio

Published inMycorrhiza, vol. 9, no. 2, p. 65-68

Publication date1999-08-23

Abstract

The effects of sterilization time, sterilizing agents (ethanol, Chloramine T, calcium hypochlorite) and antibiotics (streptomycin and gentamycin) on Glomus mosseae (BEG 12) sporocarp germination and contamination were evaluated. Incubation for 10 s in 96 % ethanol, followed by 10 min in a solution of 2% Chloramine T, 0.02% streptomycin, 0.01% gentamycin and Tween 20, and then 6 min in 6% calcium hypochlorite greatly reduced fungal and bacterial contamination from sporocarps and caused little change in germination rate in water agar medium.

Keywords

Glomus mosseae
Sporocarps
Surface sterilization
Germination

Affiliation entities

Not a UNIGE publication

Citation (ISO format)

BUDI, Sri Wilarso, BLAL, Bachar, GIANINAZZI, Silvio. Surface-sterilization of Glomus mosseae sporocarps for studying endomycorrhization in vitro. In: Mycorrhiza, 1999, vol. 9, n° 2, p. 65–68. doi: 10.1007/s005720050001

Article (Published version)

OA NATIONAL

Identifiers

PID : unige:171756
DOI : 10.1007/s005720050001

Journal ISSN0940-6360

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Surface-sterilization of Glomus mosseae sporocarps for studying endomycorrhization in vitro

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