Scientific article
OA Policy
English

Phase separation of +TIP networks regulates microtubule dynamics

First online date2023-08-21
Abstract

Regulation of microtubule dynamics is essential for diverse cellular functions, and proteins that bind to dynamic microtubule ends can regulate network dynamics. Here, we show that two conserved microtubule end-binding proteins, CLIP-170 and EB3, undergo phase separation and form dense liquid networks. When CLIP-170 and EB3 act together, the multivalency of the network increases, which synergistically increases the amount of protein in the dense phase. In vitro and in cells, these liquid networks can concentrate tubulin. In vitro, in the presence of microtubules, phase separation of EB3/CLIP-170 can enrich tubulin all along the microtubule. In this condition, microtubule growth speed increases up to twofold and the frequency of depolymerization events are strongly reduced compared to conditions in which there is no phase separation. Our data show that phase separation of EB3/CLIP-170 adds an additional layer of regulation to the control of microtubule growth dynamics.

Research groups
Citation (ISO format)
MIESCH, Julie et al. Phase separation of +TIP networks regulates microtubule dynamics. In: Proceedings of the National Academy of Sciences, 2023, vol. 120, n° 35. doi: 10.1073/pnas.2301457120
Main files (1)
Article (Published version)
Identifiers
Journal ISSN0027-8424
260views
61downloads

Technical informations

Creation25/08/2023 08:16:39
First validation25/08/2023 08:45:43
Update time25/08/2023 08:49:16
Status update25/08/2023 08:49:16
Last indexation01/11/2024 05:52:38
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack