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Scientific article
Open access
English

Extracellular matrix-derived hydrogels for dental stem cell delivery

Published inJournal of biomedical materials research. Part A, vol. 105, no. 1, p. 319-328
Publication date2017-01
First online date2016-11-05
Abstract

Decellularized mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration. Bone, spinal cord, and dentine ECM hydrogels exhibited distinct structural, mechanical, and biological characteristics. All three hydrogels supported SCAP viability and proliferation. However, only spinal cord and bone derived hydrogels promoted the expression of neural lineage markers. The specific environment of ECM scaffolds significantly affected the differentiation of SCAP to a neural lineage, with stronger responses observed with spinal cord ECM hydrogels, suggesting that site-specific tissues are more likely to facilitate optimal stem cell behavior for constructive spinal cord regeneration.

eng
Keywords
  • ECM hydrogels
  • Bone
  • Dental stem cell delivery
  • Dentine
  • Spinal cord
  • Cell Differentiation
  • Cell Line
  • Dental Papilla / cytology
  • Dental Papilla / metabolism
  • Extracellular Matrix / chemistry
  • Humans
  • Hydrogels / chemistry
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Stem Cell Niche
Affiliation Not a UNIGE publication
Citation (ISO format)
VISWANATH, Aiswarya et al. Extracellular matrix-derived hydrogels for dental stem cell delivery. In: Journal of biomedical materials research. Part A, 2017, vol. 105, n° 1, p. 319–328. doi: 10.1002/jbm.a.35901
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Article (Accepted version)
accessLevelPublic
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ISSN of the journal1549-3296
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