Scientific article
Open access

Enhancement of vitamin B6 levels in rice expressing Arabidopsis vitamin B6 biosynthesis de novo genes

Published inPlant journal, vol. 99, no. 6, p. 1047-1065
Publication date2019-09
First online date2019-07-11

Vitamin B6(pyridoxine) is vital for key metabolic reactions and reported to have antioxidant properties in planta. Therefore, enhancement of vitamin B6content has been hypothesized to be a route to improve resistance to biotic and abiotic stresses. Most of the current studies on vitamin B6in plants are on eudicot species, with monocots remaining largely unexplored. In this study, we investigated vitamin B6biosynthesis in rice, with a view to examining the feasibility and impact of enhancing vitamin B6levels. Constitutive expression in rice of two Arabidopsis thaliana genes from the vitamin B6biosynthesis de novo pathway, AtPDX1.1 and AtPDX2, resulted in a considerable increase in vitamin B6in leaves (up to 28.3-fold) and roots (up to 12-fold), with minimal impact on general growth. Rice lines accumulating high levels of vitamin B6did not display enhanced tolerance to abiotic stress (salt) or biotic stress (resistance to Xanthomonas oryzae infection). While a significant increase in vitamin B6content could also be achieved in rice seeds (up to 3.1-fold), the increase was largely due to its accumulation in seed coat and embryo tissues, with little enhancement observed in the endosperm. However, seed yield was affected in some vitamin B6-enhanced lines. Notably, expression of the transgenes did not affect the expression of the endogenous rice PDX genes. Intriguingly, despite transgene expression in leaves and seeds, the corresponding proteins were only detectable in leaves and could not be observed in seeds, possibly pointing to a mode of regulation in this organ.

  • PDX proteins
  • Crop
  • Monocot
  • Rice
  • Stress
  • Vitamin B6
  • Arabidopsis / genetics
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Bacterial Infections / genetics
  • Bacterial Infections / metabolism
  • Bacterial Infections / pathology
  • Carbon-Nitrogen Lyases / genetics
  • Carbon-Nitrogen Lyases / metabolism
  • Endosperm / metabolism
  • Gene Expression Regulation, Plant / genetics
  • Nitrogenous Group Transferases / genetics
  • Nitrogenous Group Transferases / metabolism
  • Oryza / genetics
  • Oryza / growth & development
  • Oryza / metabolism
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Plants, Genetically Modified / metabolism
  • Salt Stress / physiology
  • Seeds / metabolism
  • Transgenes
  • Vitamin B 6 / biosynthesis
  • Vitamin B 6 / metabolism
  • Xanthomonas / pathogenicity
Citation (ISO format)
MANGEL, Nathalie et al. Enhancement of vitamin B6 levels in rice expressing Arabidopsis vitamin B6 biosynthesis de novo genes. In: Plant journal, 2019, vol. 99, n° 6, p. 1047–1065. doi: 10.1111/tpj.14379
Main files (1)
Article (Published version)
Secondary files (4)
ISSN of the journal0960-7412

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