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Scientific article
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English

Mechanistic insights into the regulation of plant phosphate homeostasis by the rice SPX2 - PHR2 complex

Published inNature communications, vol. 13, no. 1, 1581
Publication date2022-03-24
First online date2022-03-24
Abstract

Phosphate (Pi) starvation response (PHR) transcription factors play key roles in plant Pi homeostasis maintenance. They are negatively regulated by stand-alone SPX proteins, cellular receptors for inositol pyrophosphate (PP-InsP) nutrient messengers. How PP-InsP-bound SPX interacts with PHRs is poorly understood. Here, we report crystal structures of the rice SPX2/InsP6/PHR2 complex and of the PHR2 DNA binding (MYB) domain in complex with target DNA at resolutions of 3.1 Å and 2.7 Å, respectively. In the SPX2/InsP6/PHR2 complex, the signalling-active SPX2 assembles into a domain-swapped dimer conformation and binds two copies of PHR2, targeting both its coiled-coil (CC) oligomerisation domain and MYB domain. Our results reveal that the SPX2 senses PP-InsPs to inactivate PHR2 by establishing severe steric clashes with the PHR2 MYB domain, preventing DNA binding, and by disrupting oligomerisation of the PHR2 CC domain, attenuating promoter binding. Our findings rationalize how PP-InsPs activate SPX receptor proteins to target PHR family transcription factors.

eng
Keywords
  • Gene Expression Regulation, Plant
  • Homeostasis
  • Oryza / genetics
  • Oryza / metabolism
  • Phosphates / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants / metabolism
  • Transcription Factors / metabolism
Funding
  • European Commission - System-wide discovery and analysis of inositol pyrophosphate signaling networks in plants [818696]
Citation (ISO format)
GUAN, Zeyuan et al. Mechanistic insights into the regulation of plant phosphate homeostasis by the rice SPX2 - PHR2 complex. In: Nature communications, 2022, vol. 13, n° 1, p. 1581. doi: 10.1038/s41467-022-29275-8
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Identifiers
ISSN of the journal2041-1723
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