Scientific article
Open access

Natural Variation in Vitamin B1 and Vitamin B6 Contents in Rice Germplasm

Published inFrontiers in plant science, vol. 13, 856880
Publication date2022
First online date2022-04-04

Insufficient dietary intake of micronutrients contributes to the onset of deficiencies termed hidden hunger-a global health problem affecting approximately 2 billion people. Vitamin B1(thiamine) and vitamin B6(pyridoxine) are essential micronutrients because of their roles as enzymatic cofactors in all organisms. Metabolic engineering attempts to biofortify rice endosperm-a poor source of several micronutrients leading to deficiencies when consumed monotonously-have led to only minimal improvements in vitamin B1and B6contents. To determine if rice germplasm could be exploited for biofortification of rice endosperm, we screened 59 genetically diverse accessions under greenhouse conditions for variation in vitamin B1and vitamin B6contents across three tissue types (leaves, unpolished and polished grain). Accessions from low, intermediate and high vitamin categories that had similar vitamin levels in two greenhouse experiments were chosen for in-depth vitamer profiling and selected biosynthesis gene expression analyses. Vitamin B1and B6contents in polished seeds varied almost 4-fold. Genes encoding select vitamin B1and B6biosynthesisde novoenzymes (THICfor vitamin B1,PDX1.3a-candPDX2for vitamin B6) were differentially expressed in leaves across accessions contrasting in their respective vitamin contents. These expression levels did not correlate with leaf and unpolished seed vitamin contents, except forTHICexpression in leaves that was positively correlated with total vitamin B1contents in polished seeds. This study expands our knowledge of diversity in micronutrient traits in rice germplasm and provides insights into the expression of genes for vitamin B1and B6biosynthesis in rice.

  • Biofortification
  • Germplasm
  • Hidden hunger
  • Micronutrient deficiency
  • Natural variation
  • Rice
  • Vitamin B1
  • Vitamin B6
Citation (ISO format)
MANGEL, Nathalie et al. Natural Variation in Vitamin B1 and Vitamin B6 Contents in Rice Germplasm. In: Frontiers in plant science, 2022, vol. 13, p. 856880. doi: 10.3389/fpls.2022.856880
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ISSN of the journal1664-462X

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